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plvpt ttr krab  (Addgene inc)


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    Structured Review

    Addgene inc plvpt ttr krab
    Plvpt Ttr Krab, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plvpt+ttr+krab/pLVPT-tTR-KRAB+(Plasmid+%2311642)/us12338443-301-45-50
    Average 92 stars, based on 9 article reviews
    plvpt ttr krab - by Bioz Stars, 2026-09
    92/100 stars

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    Related Articles

    Transfection:

    Article Title: Doxycycline-regulated GDNF expression promotes axonal regeneration and functional recovery in transected peripheral nerve.
    Article Snippet: a Department of Clinical Neuroscience, Faculty of Medicine, University of Calgary, Calgary, Canada b Hotchkiss Brain Institute, University of Calgary, Calgary, Canada c Institute of Biophysics and Cell Engineering, National Academy of Sciences of Belarus, Minsk, Belarus d Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, Lodz, Poland e Laboratoire de Chimie de Coordination, CNRS, Toulouse, France

    Plasmid Preparation:

    Article Title: Doxycycline-regulated GDNF expression promotes axonal regeneration and functional recovery in transected peripheral nerve.
    Article Snippet: a Department of Clinical Neuroscience, Faculty of Medicine, University of Calgary, Calgary, Canada b Hotchkiss Brain Institute, University of Calgary, Calgary, Canada c Institute of Biophysics and Cell Engineering, National Academy of Sciences of Belarus, Minsk, Belarus d Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, Lodz, Poland e Laboratoire de Chimie de Coordination, CNRS, Toulouse, France

    Article Title: Light Control of the Tet Gene Expression System in Mammalian Cells.
    Article Snippet: .. For functional screening of PA-Tet-OFF candidate constructs, sequences encoding the DNA binding, dimerization, and Tet binding domains of TetR (residues 1–206), and the transcriptional activation domain of p65 were amplified using pLVPT-tTR-KRAB (Addgene plasmid #11642) (Szulc et al., 2006) and pEF-hGAVPO (Imayoshi et al., 2013;Wang et al., 2012), respectively. ..

    Article Title: Photoactivatable Tet expression control system
    Article Snippet: .. For functional screening of PA-Tet-OFF candidate constructs, a DNA binding domain, a dimerization domain, and a Tet binding domain (residues 1 to 206 of TetR, hereinafter, represented by “TetR (1-206)”) of TetR (SEQ ID NO: 1) and p65AD (SEQ ID NO: 2) were amplified using pLVPT-tTR-KRAB (plasmid #11642, manufactured by Addgene) (Non-Patent Literature 12) and pEF-hGAVPO (Non-Patent Literatures 2 and 13), respectively. .. The nucleic acids having sequences optimized for the mammalian codons encoding Cry2 (SEQ ID NO: 3) variants (Cry2 PHR, Cry2 PHR (L348F), Cry2 535, and Cry2 535 (L348F)), CIB1 (SEQ ID NO: 4), and variants thereof (CIB1 without a nuclear localization sequence [NLS], CIBN, CIBN without NLS, and CIB81) were synthesized by FASMAC (Non-Patent Literatures 10, 11, and 14).

    Expressing:

    Article Title: Doxycycline-regulated GDNF expression promotes axonal regeneration and functional recovery in transected peripheral nerve.
    Article Snippet: a Department of Clinical Neuroscience, Faculty of Medicine, University of Calgary, Calgary, Canada b Hotchkiss Brain Institute, University of Calgary, Calgary, Canada c Institute of Biophysics and Cell Engineering, National Academy of Sciences of Belarus, Minsk, Belarus d Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, Lodz, Poland e Laboratoire de Chimie de Coordination, CNRS, Toulouse, France

    Introduce:

    Article Title: PPARgamma dependent PEX11beta counteracts the suppressive role of SIRT1 on neural differentiation of HESCs.
    Article Snippet: .. We employed lentiviral vectors, including pLVTHM and pLVPT-tTR-KRAB (Addgene), to introduce short hairpin RNA (shRNA) targeting PEX11B into the genome of hESCs, following the supplier’s protocol (Addgene, Cambridge, MA, USA; Table 1). ..

    Article Title: PPARgamma dependent PEX11beta counteracts the suppressive role of SIRT1 on neural differentiation of HESCs
    Article Snippet: .. We employed lentiviral vectors, including pLVTHM and pLVPT-tTR-KRAB (Addgene), to introduce short hairpin RNA (shRNA) targeting PEX11B into the genome of hESCs, following the supplier’s protocol (Addgene, Cambridge, MA, USA; ). ..

    shRNA:

    Article Title: PPARgamma dependent PEX11beta counteracts the suppressive role of SIRT1 on neural differentiation of HESCs.
    Article Snippet: .. We employed lentiviral vectors, including pLVTHM and pLVPT-tTR-KRAB (Addgene), to introduce short hairpin RNA (shRNA) targeting PEX11B into the genome of hESCs, following the supplier’s protocol (Addgene, Cambridge, MA, USA; Table 1). ..

    Article Title: PPARgamma dependent PEX11beta counteracts the suppressive role of SIRT1 on neural differentiation of HESCs
    Article Snippet: .. We employed lentiviral vectors, including pLVTHM and pLVPT-tTR-KRAB (Addgene), to introduce short hairpin RNA (shRNA) targeting PEX11B into the genome of hESCs, following the supplier’s protocol (Addgene, Cambridge, MA, USA; ). ..

    Functional Assay:

    Article Title: Light Control of the Tet Gene Expression System in Mammalian Cells.
    Article Snippet: .. For functional screening of PA-Tet-OFF candidate constructs, sequences encoding the DNA binding, dimerization, and Tet binding domains of TetR (residues 1–206), and the transcriptional activation domain of p65 were amplified using pLVPT-tTR-KRAB (Addgene plasmid #11642) (Szulc et al., 2006) and pEF-hGAVPO (Imayoshi et al., 2013;Wang et al., 2012), respectively. ..

    Article Title: Photoactivatable Tet expression control system
    Article Snippet: .. For functional screening of PA-Tet-OFF candidate constructs, a DNA binding domain, a dimerization domain, and a Tet binding domain (residues 1 to 206 of TetR, hereinafter, represented by “TetR (1-206)”) of TetR (SEQ ID NO: 1) and p65AD (SEQ ID NO: 2) were amplified using pLVPT-tTR-KRAB (plasmid #11642, manufactured by Addgene) (Non-Patent Literature 12) and pEF-hGAVPO (Non-Patent Literatures 2 and 13), respectively. .. The nucleic acids having sequences optimized for the mammalian codons encoding Cry2 (SEQ ID NO: 3) variants (Cry2 PHR, Cry2 PHR (L348F), Cry2 535, and Cry2 535 (L348F)), CIB1 (SEQ ID NO: 4), and variants thereof (CIB1 without a nuclear localization sequence [NLS], CIBN, CIBN without NLS, and CIB81) were synthesized by FASMAC (Non-Patent Literatures 10, 11, and 14).

    Construct:

    Article Title: Light Control of the Tet Gene Expression System in Mammalian Cells.
    Article Snippet: .. For functional screening of PA-Tet-OFF candidate constructs, sequences encoding the DNA binding, dimerization, and Tet binding domains of TetR (residues 1–206), and the transcriptional activation domain of p65 were amplified using pLVPT-tTR-KRAB (Addgene plasmid #11642) (Szulc et al., 2006) and pEF-hGAVPO (Imayoshi et al., 2013;Wang et al., 2012), respectively. ..

    Article Title: Photoactivatable Tet expression control system
    Article Snippet: .. For functional screening of PA-Tet-OFF candidate constructs, a DNA binding domain, a dimerization domain, and a Tet binding domain (residues 1 to 206 of TetR, hereinafter, represented by “TetR (1-206)”) of TetR (SEQ ID NO: 1) and p65AD (SEQ ID NO: 2) were amplified using pLVPT-tTR-KRAB (plasmid #11642, manufactured by Addgene) (Non-Patent Literature 12) and pEF-hGAVPO (Non-Patent Literatures 2 and 13), respectively. .. The nucleic acids having sequences optimized for the mammalian codons encoding Cry2 (SEQ ID NO: 3) variants (Cry2 PHR, Cry2 PHR (L348F), Cry2 535, and Cry2 535 (L348F)), CIB1 (SEQ ID NO: 4), and variants thereof (CIB1 without a nuclear localization sequence [NLS], CIBN, CIBN without NLS, and CIB81) were synthesized by FASMAC (Non-Patent Literatures 10, 11, and 14).

    Binding Assay:

    Article Title: Light Control of the Tet Gene Expression System in Mammalian Cells.
    Article Snippet: .. For functional screening of PA-Tet-OFF candidate constructs, sequences encoding the DNA binding, dimerization, and Tet binding domains of TetR (residues 1–206), and the transcriptional activation domain of p65 were amplified using pLVPT-tTR-KRAB (Addgene plasmid #11642) (Szulc et al., 2006) and pEF-hGAVPO (Imayoshi et al., 2013;Wang et al., 2012), respectively. ..

    Article Title: Photoactivatable Tet expression control system
    Article Snippet: .. For functional screening of PA-Tet-OFF candidate constructs, a DNA binding domain, a dimerization domain, and a Tet binding domain (residues 1 to 206 of TetR, hereinafter, represented by “TetR (1-206)”) of TetR (SEQ ID NO: 1) and p65AD (SEQ ID NO: 2) were amplified using pLVPT-tTR-KRAB (plasmid #11642, manufactured by Addgene) (Non-Patent Literature 12) and pEF-hGAVPO (Non-Patent Literatures 2 and 13), respectively. .. The nucleic acids having sequences optimized for the mammalian codons encoding Cry2 (SEQ ID NO: 3) variants (Cry2 PHR, Cry2 PHR (L348F), Cry2 535, and Cry2 535 (L348F)), CIB1 (SEQ ID NO: 4), and variants thereof (CIB1 without a nuclear localization sequence [NLS], CIBN, CIBN without NLS, and CIB81) were synthesized by FASMAC (Non-Patent Literatures 10, 11, and 14).

    Activation Assay:

    Article Title: Light Control of the Tet Gene Expression System in Mammalian Cells.
    Article Snippet: .. For functional screening of PA-Tet-OFF candidate constructs, sequences encoding the DNA binding, dimerization, and Tet binding domains of TetR (residues 1–206), and the transcriptional activation domain of p65 were amplified using pLVPT-tTR-KRAB (Addgene plasmid #11642) (Szulc et al., 2006) and pEF-hGAVPO (Imayoshi et al., 2013;Wang et al., 2012), respectively. ..

    Amplification:

    Article Title: Light Control of the Tet Gene Expression System in Mammalian Cells.
    Article Snippet: .. For functional screening of PA-Tet-OFF candidate constructs, sequences encoding the DNA binding, dimerization, and Tet binding domains of TetR (residues 1–206), and the transcriptional activation domain of p65 were amplified using pLVPT-tTR-KRAB (Addgene plasmid #11642) (Szulc et al., 2006) and pEF-hGAVPO (Imayoshi et al., 2013;Wang et al., 2012), respectively. ..

    Article Title: Photoactivatable Tet expression control system
    Article Snippet: .. For functional screening of PA-Tet-OFF candidate constructs, a DNA binding domain, a dimerization domain, and a Tet binding domain (residues 1 to 206 of TetR, hereinafter, represented by “TetR (1-206)”) of TetR (SEQ ID NO: 1) and p65AD (SEQ ID NO: 2) were amplified using pLVPT-tTR-KRAB (plasmid #11642, manufactured by Addgene) (Non-Patent Literature 12) and pEF-hGAVPO (Non-Patent Literatures 2 and 13), respectively. .. The nucleic acids having sequences optimized for the mammalian codons encoding Cry2 (SEQ ID NO: 3) variants (Cry2 PHR, Cry2 PHR (L348F), Cry2 535, and Cry2 535 (L348F)), CIB1 (SEQ ID NO: 4), and variants thereof (CIB1 without a nuclear localization sequence [NLS], CIBN, CIBN without NLS, and CIB81) were synthesized by FASMAC (Non-Patent Literatures 10, 11, and 14).



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